eclipse ti e c2 confocal laser scanning microscope  (Nikon)

Journal: Biofilm

Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

doi: 10.1016/j.bioflm.2025.100335

Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

Article Snippet: After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses.

Techniques: Staining, Mutagenesis, Fluorescence, Microscopy

Journal: bioRxiv

Article Title: Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation

doi: 10.64898/2026.03.19.712761

Figure Lengend Snippet: (A) Schematic overview of the experimental design and analytical approaches used in this study. (B) FAF images showing that Lamp2a loss leads to accumulation of high-intensity autofluorescence spots, which are significantly reduced by GLA-1-1 treatment. (C) Quantification of high-intensity autofluorescence spots (WT (n=11), Lamp2a -/- mice (n=11), Lamp2a -/- + GLA-1-1 mice (n=18)). (D) OCT B-scans of WT, Lamp2a -/- untreated, and Lamp2a -/- -GLA-1-1-treated mouse eyes. GLA-1-1 treatment prevented RPE hyperplasia and extension of dysmorphic RPE cells into the ONL. (E) Autofluorescence images of retinal sections showing accumulation autofluorescent granules in Lamp2a -/- as compared to WT mice. GLA-1-1 treatment significantly reduced the accumulation of the granules in RPE of Lamp2a -/- mice. The images were captured with confocal microscope using the excitation wavelength of 488 nm and emission wavelengths of 500–600 nm. (F) Quantification of fluorescence intensity of autofluorescence granules (n=7). Statistical analysis was performed using one-way ANOVA with post hoc Tukey test for C and F ***P < 0.001; ****P < 0.0001. Values are expressed as mean ± SD.

Article Snippet: Sections were washed again three times with PBS, mounted using Vectashield antifade mounting medium containing DAPI (Vector Laboratories), and imaged using a Nikon Ti Eclipse confocal microscope equipped with a ×40 oil-immersion objective.

Techniques: Microscopy, Fluorescence